GC-MS Analysis of Phytocomponents in the Various Extracts of Shorea robusta Gaertn F.
Vashisht Sushma, Singh Manish Pal, Chawla Viney*
Rajiv Academy for Pharmacy, N.H. #2, Delhi Mathura Bye Pass, P.O. Chattikara, Mathura-281003, Uttar Pradesh, India.
Received: 28th May, 17; Revised and Accepted: 15th June, 17; Available Online:25th June, 2017
The current investigation was carried out to determine the possible phytocomponents present in the different extracts of Shorea robusta using gas chromatography-mass spectrometry (GC-MS). The dried powdered resin of Shorea robusta was extracted exhaustively by Soxhlet apparatus with different solvents such as methanol, ethanol and chloroform. The prepared extracts were analyzed by GC-MS to identify and characterize the phytocomponents present in the crude extracts. Qualitative determination of different phytocomponents from crude extracts of Shorea robusta using GC-MS revealed different types of high and low molecular weight phytoconstituents with varying quantities present in each of the extracts. The GC-MS analysis provided a variety of peaks determining the presence of different compounds in various extracts of Shorea robusta namely Caryophyllene (1.50%), Caryophylline oxide (6.65%), Ledene oxide (11.17%), Calarene epoxide (5.15%), Alloaromadendrene oxide-(1) (8.72%), Beta-amyrin (7.99%), Alpha-amyrin (1.40%), Cycloisolongifolene (2.54%), Isolongifolene (4.73%), Silane (2.64%). The three extracts possess major phytoconstituents that were identified and characterized spectroscopically. The abundance of phytoconstituents was found to decrease in the order: methanol extract>ethanol extract>chloroform extract.
Keywords: GC-MS analysis, Shorea robusta, Phytocomponents, Caryophyllene.