Phytochemical Screening, HPTLC Fingerprint Analysis of Leaf Extract of Bridelia montana (Roxb) Willd
B Vinatha* , Vaishali Kute, Vijaya Kuchana, J Shreelekha, K Sreeharshini, I Anvesh Reddy
Teegala Krishna Reddy College of Pharmacy, Medbowli, Meerpet, Saroornagar, Hyderabad, Telangana, India
Received: 13th April, 17; Revised 23rd May, 17, Accepted: 15th June, 17; Available Online:25th June, 2017
This research analyzed the major chemical constituents present in the ethanolic extract of the leaves of Bridelia montana (ROXB) WILLD. The leaves of Bridelia montana were collected, powdered, de-fatted with n-hexane and then extracted with 90%ethyl alcohol. Preliminary phytochemical screening of the ethanolic leaf extract of Bridelia montana showed the presence of chemical constituents like alkaloids, glycosides, phenolic compounds, tannins and resins. HPTLC fingerprint analysis of the extract was carried out using CAMAG HPTLC system equipped with Linomat 5 sample applicator, UV cabinet and WIN-CATS-4 software. The results from HPTLC fingerprint analysis of the ethanolic leaf extract of Bridelia montana revealed the presence of 6 polyvalent constituents.
Keywords: Bridelia montana (ROXB) WILLD. HPTLC, CAMAG, WIN CATS-4.