Determination of Total Phenolic, Tannin, Flavonoid Contents and Evaluation of Antioxidant Property of Amaranthus tricolor (L)
Sowjanya Pulipati*, P Srinivasa Babu, U Naveena, S K Rafeeka Parveen, S K Sumaya Nausheen, M Tanmai Naga Sai
Department of Biotechnology, Vignan Pharmacy College, Vadlamudi-522213, Andhra Pradesh, India
Received: 24th April, 17; Revised 8th June, 17, Accepted: 15th June, 17; Available Online:25th June, 2017
Free radicals or reactive oxygen species are involved in various pharmacological conditions. As synthetic antioxidants possess numerous adverse health effects, the medicinal plants possessing antioxidant components can be used to prevent harmful effects of reactive oxygen species. In the present study leaves of Amaranthus tricolor Linn were used to prepare chloroform (CEAT), methanolic (MEAT) and aqueous (AEAT) extracts, analyze the presence of phytochemicals and evaluation of in-vitro antioxidant property. Quantitative determination of phenols, tannins and flavonoids in leaves A.tricolor was carried out using spectrophotometric methods. The antioxidant activity was performed by DPPH, p-NDA radical scavenging methods for different extracts of the plant. The plant species showed that methanolic extract (MEAT) on higher concentration possess better antioxidant potential when compared with reference standard ascorbic acid. The plant extracts exhibited strong antioxidant DPPH radical scavenging activity with the IC50 values 290, 657, 830 & 130μg/ml of MEAT, CEAT, AEAT & ASA respectively. In scavenging hydroxyl radical by p-NDA method the MEAT showed maximum activity, CEAT showed moderate and AEAT showed minimum activity. The strongest antioxidant activity of MEAT could be due to the presence of flavonoids and phenols.
Keywords: Amaranthus tricolor, phenols, flavonoids, DPPH, p-NDA.