Studies on Analysis of Antioxidant and Enzyme Inhibitory Activity of Vitex negundo Linn.

Ved Prakash*, Shelly Rana, Anand Sagar

Department of Biosciences, Himachal Pradesh University, Shimla, (H.P.) 171005, India 

Received: 19th April, 17; Revised 3rd June, 17, Accepted: 15th June, 17; Available Online:25th June, 2017 


The current study was designed to investigate the leaf extracts of Vitex negundo Linn. for their antioxidant and enzyme inhibitory (α-amylae and urease) activity. The antioxidant capacity of the different extracts (methanol, acetone and aqueous) of this plant was evaluated by DPPH (1,1-diphenyl-2-picrylhydrazyl) and reducing power tests. The plant exhibited good DPPH radical scavenging activity and moderate reducing power potential Further, all the extracts of V. negundo were reported to possess good anti-alpha amylase and anti-urease activity of greater than 50% in all the solvents used at a concentration of 1 mg/mL. Thus the study provided scientific evidence to the traditional uses of this plant in the treatment of obesity, diabetes, ulcers, kidney stones etc. Therefore, the leaf extracts of this plant can be selected for further investigation to determine their therapeutic potential.

Keywords: Vitex negundo, leaf extracts, DPPH, reducing power, α-amylase, urease.

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International Journal of Pharmacognosy and Phytochemical Research