In-Vitro Antidiabetic Activity of Clinacanthus nutans Extracts
Abdullah N, Kasim K F*
School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia
Received: 17th March, 17; Revised: 20th May, 17; Accepted: 15th June, 17; Available Online:25th June, 2017
Diabetes mellitus is a prevalent disease which characterized by hyperglycemia. It is a condition in which blood glucose levels are elevated due to decrease in cellular glucose uptake and metabolism. The management of blood glucose level is critical in the treatment of this disease and this had been offered by α-amylase inhibitors. The present study was designed to determine the in vitro antidiabetic of Clinacanthus nutans extracts. The antidiabetic action was observed by the inhibition effects of the C. nutans extracts on α-amylase activity and glucose diffusion across the dialysis tube. It was found that the antidiabetic action of the C. nutans extracts was not related to glucose diffusion as they did not show any significant glucose entrapment ability. The ethanolic leaves extract of C. nutans exhibited the most significant inhibition effect on α-amylase activity (64.25%) compared to other extracts. However, its inhibitory activity was moderate when compared to the commercial drugs (captopril & acarbose). The ethanolic leaves extract (the best extract) was tested for the presence for flavonoids, saponins and tannin (most reported antidiabetic compounds). Its antidiabetic action might be due to the presence of flavonoids and tannins. However, the absence of saponins might be responsible for its moderate inhibition effect comparable to control. This study suggested the in vivo studies of this plant should be carried out to confirm its antidiabetic mechanism.
Keywords: Clinacanthus nutans, antidiabetic activity