Evaluation of Anti-Tumour Potential of Acanthus ilicifolius (Linn.) in HepG2 Cell Line Induced Hepatocellular Carcinoma in Mice
R Vijayaraj, N Sri Kumaran*, Swarnakala
Department of Marine Biotechnology, AMET University, Kanathur, Chennai – 603112
Received: 28th March, 17; Revised 7th June, 17, Accepted: 15th June, 17; Available Online:25th June, 2017
Acanthus ilicifolius has long history of medicinal plant belong to the family Acanthaceae. In the present study, ethanolic extract of A. ilicifolius showed antitumour potential against HepG2 cell line induced hepatocarcinoma in mice. The phytochemical analysis were studied by using strandard methods. The heptocarcinoma mice were orally treated with extract of A.ilicifolius (500 mg/kg B.W). The result indicates the presence of phytoconstituents such as Protein (2.5%), Carbohydrates (0.16%) , alkaloids (0.42%), flavonoids (0.62%), saponins (4.5%), tannins (1.0%) and terpenoids (0.20%). In FTIR spectrum of test sample, the strong absorbance were showed at peak 678.94 cm-1, 1514.12 cm-1,1741.72 cm-1, 2304.94 cm-1, 3597.24 cm-1, 3728.40 cm-1 which are associated with presence of functional groups such as Mono substituted benzene, aromatic (c=c), aldehyde (c=o), disubstituted alkynes(c=c), alcohols with free hydroxyl group (O-H), alcohols, phenols (O-H). Treatment effectively suppressed HepG2 cells induced mice as revealed by decrease in the levels and extend of Alanine transaminase (84.3±1.86), Aspartate transaminase (41.6±2.15), Alkaline phosphatase (224.9±6.3), Urea (64.3±1.20) and creatinine (1.36±0.155). Histopathological analysis of liver and kidney was also evaluated. This study demonstrates that the A. ilicofolius have a potent anticancer Properties.
Keywords: Acanthus ilicifolius, HepG2 cells, Hepatocellular Carcinoma, Anticancer.