1. Total Antioxidant Profile of some Common Medicinal Plants of West Bengal Puspal De, Samipa Das, Madhumita J Mukhophadhyay
Background: Nature provides several phyto constituents since thousands of years and many of them are used as an alternative drug in Ayurvedic and folk medicine.The medicinal value greatly depends on their antioxidant property. So, these herbal extracts are essentially important for the remediation of reactive oxygen species. Aim: Determination of total antioxidant property of twelve common Indian plant extract used in our daily life for several ways. Method: The crude aqueous extract was prepared and then phospo-molybdenum method was adopted for the study of total antioxidant property. Result: Among twelve common plant extracts bramhi stands first for its antioxidant property and rest of them are depicted in their decreasing order – green tea, sajne, laal sag, basaka, coffee, black tea, pineapple, turmeric, licorice, betel leaf. Conclusion: A number of common plants, known to have medicinal values were assayed for their antioxidant property. Bramhi shows maximum efficacy in this respect, whereas all other medicinal plants assayed showed antioxidant property to some extent.
2. Spectral Studies and Biochemical Evaluation of Citrus limon Pulp Rangarajan N, Sampath V, Dass Prakash M V
Novel chemical compounds synthesized from the active constituents of plants are of potential use in medicine and other useful applications. Citrus limon fruits have long been valued as part of a nutritious and tasty diet. Flavonoids are the important constituents present in the Citrus limon fruits. Flavonoids have gained recent attention because of their broad biological and pharmacological activities. The objective of this study was to identify the phytochemicals and to analyse flavonoids in the Citrus limon pulp. The crude methanol and chloroform extracts of Citrus limon were subjected to preliminary screening. The Phytochemical screening of the Citrus limon pulp extracts revealed the presence of polyphenols, alkaloids, flavanoids, terpenoids, amino acids and proteins. The DPPH free radical scavenging activity of Citrus limon pulp tissue shows a significant antioxidant property. The HPLC studies indicate that the quercetin is the major flavonoid present in the Citrus limon pulp juice. FT-IR and UV Spectral studies of Citrus limon pulp show the presence of different flavanoids in the Citrus limon pulp.
3. Total Phenolic, Total Flavonoid Content, Two Isolates and Bioactivity of Lavandula pubescens Decne Mousa O, Gouda B, Salama M, El-Eraky W, Kassem H
The phenolics and flavonoids content of Lavandula pubescens Decne. cultivated in Egypt were determined. The phenolic content of the ethanolic extract of the flowering aerial parts of the plant was measured by using Folin-Ciocalteu assay colourimetric method. The total phenolic content was expressed as 234.17±0.37 µg GAE/mg of sample. The total flavonoids of the extract were measured spectrophotometrically by using aluminium chloride colorimetric assay. The percentage of flavonoids was calculated as 134.36±0.067 µg quercetin/ mg of sample. Two compounds were isolated from the ethyl acetate fraction and identified on basis of their 1H-NMR and 13C-NMR as acacetin-7-O- glucoside and gallic acid. Significant biological assessment of the three tested concentrations of the extract was obtained applying the carrageenan-induced rat paw oedema and hot plate method as models for acute anti-inflammatory and analgesic effects respectively. A remarkable hepatoprotective activity of the extract as regards to silymarin, against paracetamol induced acute liver damage in rat was observed. A considerable decrease in the serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was recorded. Liver histopathology of the methanolic extract treated rats exhibited significant protection against paracetamol intoxication.
4. Antioxidant, Anti-inflammatory and Antinociceptive Activities of Essential Oils of Three Satureja Species (S. briquetti, S. atlantica and S. alpina) Growing Wild in the Middle Atlas Mountains of Morocco Jennan S, Fouad R, Farah A, Bousta D, Mahjoubi F
This article describes the biological activities of essential oils of three species of Satureja. The choice of plants and activities studied was motivated by the frequent traditional use of S. abriquetti, S. atlantica and S. alpina by indigenous populations for the efficacy and virtues they discovered. The aim of the experiments is to prove the antioxidant, anti-inflammatory and antinociceptive activities of the essential oils of S. briquetti, S. atlantica and S. alpina. The antioxidant activity was evaluated by three different methods namely the free radical scavenging, the Ferric Reducing Antioxidant Power and the phosphomolybdenum method. The Carrageenan induced paw oedema in rats assay was used to evaluate the anti-inflammatory activity of essential oils. In order to prove the antinociceptive activity, the Plantar model was chosen. The three essential oils have interesting antioxidant powers, especially by the reductive and phosphomolybdenum methods where the activities of S. atlantica and S. alpina exceed that of S. briquetti. In order to evaluate the anti-inflammatory activities of essential oils, the dose used orally is 200mg / kg. S. alpina represents the best anti-inflammatory profile with an inhibitory capacity of 91.4%. The essential oil of S. atlantica has a slightly higher antalgic power followed by S. briquetti and S. alpina respectively.The results obtained confirm the traditional therapeutic effects and the use of these plants by the autochthonous population of the Middle Atlas Mountains of Morocco.