The presence of oestrogen and progesterone receptors in breast cancer cell lines can be exploited to find the modulatory potential of phytochemicals on steroid hormone synthesis in MCF -7 cell lines. The current study was conducted to examine the modulatory potential methanolic extract of Malllotus philippensis
leaves on steroid hormone synthesis in MCF-7 breast cancer cell line. The locally collected leaves of M. phillippensis
were shade dried, pulverized and extracted using methanol in a Soxhlet apparatus, which was concentrated and stored. The phytochemicals were identified qualitatively. MTT assay was carried out to assess the in vitro
cytotoxic effects of methanolic extract of M. philippensis
in MCF-7 cells at dosage rates of 320, 160, 80, 40, 20, 10 µg/mL. From the results of MTT assay, per cent cell viability, inhibition and IC50
were determined. The cells at concentration 1×105
cells/mL were seeded in 6 well plate and incubated with the extract at doses of IC50
, half and twice IC50
for 96 hrs, media was collected after 48 and 96hrs and used to quantify the concentration of oestrogen and progesterone using ELISA. Alkaloids, tannins, flavonoids, steroids, diterpenes and phenols were identified by qualitative phytochemical analysis of extract of M. philippenisis.
The MTT assay revealed a dose dependent cytotoxicity of the extract in MCF-7 cell lines with an IC50
of 190µg/mL. Methanolic extract of M. phillippensis
showed a dose and time dependent increase in oestrogen concentration as well as a dose dependent decrease in progesterone concentrations in the culture media. Based on the findings of the present study, M. phillippensis
induced a positive modulation of oestrogen production which might be the reason for low progesterone levels.