1. Invitro Cytotoxic Activity of Ethanolic Extract OF Reissantia indica on the Human Colon Cancer Cell Line (HT-29) Sangeetha M, Sudhakar K, Sathish S, Gayathri P, Chamundeeswari D
Reissantia indica belongs to the family Celastraceae shrub, in dryish forest or wood land or on river banks, often in stony ground of Senegal to Cameroun and occurring south in the transvaalin tropical Asia from India and Ceylon to the Philippines at altitude range of 210–1170 m. Root bark is used for the treatment of respiratory troubles. Stems are considered as febrifuge. Leaves are scorched and given to women during confinement. Powdered leaves and roots are applied to sores and wounds. Effect of anticancer activity was studied on HT- 29 cell lines by MTT assay using the ethanolic extract of Reissantia indica . Different doses of plant extract and standard were taken and introduced into cancer cells were recorded at 24 hrs respectively it clearly showed us the dose dependent response for the inhibition of cells. The most potent anticancer activity has been shown at the concentration 1000 μg/mL of Reissantia indica. extract on HT-29 colon cancer cell line.
2. Phytochemical and Phytoelemental Profile of J. officinale Dubey Prachee, Tiwari Ayushi, Tiwari Praveen, Awasthi Shikha, Rai A K, Watal Geeta
Recently, plant products have started gaining importance due to their significant contribution in tackling various health issues. The medicinal prominence of these plants lies in chemical constituents present in it, termed as “phytochemicals”. Phytochemicals are naturally occurring secondary metabolites derived from plants which may be biologically active. Several major groups of phytochemicals such as flavonoids, glycosides, tannins, coumarins, terpenoids, cinnamic acids, and alkaloids etc. have been reported to possess specific bioactivities. In addition to these chemical components, “phytoelements” present in plants are also responsible for their medicinal properties either collectively or individually. Hence, detection of phytoelements is equally important in addition to the identification of phytochemicals, in order to correlate their presence as well as their synergistic impact on therapeutic efficacy of a particular medicinal plant associated with specific bioactivity. Thus, the present study deals with the phytochemical studies of J. officinale leaves in five different solvents with increasing polarity viz. hexane, chloroform, ethanol, methanol and water. Preliminary phytochemical screening of these fractions showed the presence of alkaloids, carbohydrates, coumarins, flavonoids, saponins, tannins and terpenoids. Though, the results reveal that the aqueous fraction has the maximum phytochemicals, qualitatively as well as quantitatively. Laser-induced breakdown spectroscopy (LIBS) was used for detection of elements present in aqueous fraction of J. officinale leaves and the spectral analysis indicated the significant presence of Potassium (K), Sodium (Na), Calcium (Ca) and Magnesium (Mg). Whereas, Oxygen (O) and Nitrogen (N) were present to a lesser extent comparatively. Very encouraging results are in hand on correlating the presence of these phytochemicals, and phytoelements with the specific therapeutic efficacies of J. officinale leaves and hence its aqueous fraction could be explored further as a novel therapeutic agent.