Volume10,Issue11

S.marcescens is opportunistic pathogens that cause nosocomical infections. There have been many reports concerning the identification, antibiotic susceptibility, pathogenicity, epidemiological investigations and typing of this organism. Accurate identification is important in defining outbreaks. In the present study, S. marcescens have been selected from the natural sources and were then confirmed with 16S rRNA identification method with cultures of MTCC. To investigate these S. marcescens isolates, random amplification of polymorphic DNA (RAPD) was used in conjunction with the specific primers. The RAPD patterns for each isolate were identified on the basis of identical numbers and sizes of the bands. And by using dendrogram we can able to show, that the pattern of the primers to separate isolates was closely related to their sequence homology with the genome and their amount of guanine and cytosine nucleotide content. S. marcescens as a pathogen shows a tissue-damaging capacity. The cytotoxic activity is mainly elicited by the secreted hemolysin/cytotoxin ShlA. Here, the isolates were tested for hemolytic activity. S. marcescens is known to be frequently resistant to various antibiotics and our results also showed such a tendency.

India is one of the world’s twelve leading biodiversity centers having over 45,000 different plant species. Many of these plant species have beneficial values and are used for several years by our ancestors as effective home remedies or remedies suggested by the vaidya’s or hakim’s. These ethnoplants can be considered as a great source of therapeutic agents.  However, the biggest constraint is that they have not been studied systematically and scientifically. Thus, an understanding of the pharmacognostical, pharmacological and phytochemical potential of these ethnomedicinal plants would provide an authentic data which would enable such precious source of medicinal plants to fulfill demands of the global market. Bridelia crenulata Roxb belonging to family Euphorbiaceae is one such ethnoplant, which is known to be used by the inhabitants of Orissa to prevent pregnancy. In this research article, pharmacognosy of Bridelia crenulata is studied, which may serve as a valuable source of information and provide suitable standards to determine the quality, purity and authenticity of Bridelia crenulata in future investigations or applications.