Volume2,Issue4

Anti oxidant, anti inflammatory, anti obesity and anti microbial activities in four different species of Caralluma, include Boucerosea lasiantha (BL), Caralluma adscendens var. attenuata (CAA), C.stalagmifera (CSF) and C.longipetala (CL) were investigated. Whole plant is used for the extraction of compound by using methanol. These selected species of Caralluma are used for the study of saponins and flavanoids, their bioactive nature. Variations in the bioactivity of cactus species are compared as a preliminary survey for future pharmacological drug development. Methanolic extracts of CAA exhibited highest free radical scavenger activity (FRSA) followed by BL, CSF, CL respectively with IC50 values 50,37,32, 27 μg/ml. CAA also exhibited highest anti inflammatory activity (5-Lox assay) followed by BL, CL, CSF with IC50 values 27 ,17, 12.8, 11.8 μg/ml. Methanolic extracts of four species also show anti diabetic activities (α- amylase property assay). Extracts of four species show anti microbial activity, however CSF and CL show highest activity than CAA and BL. These results suggest that the methanol extract of Caralluma species show significant anti oxidant, anti inflammatory, anti diabetic, anti microbial activities, which could be used as a potential source of pharmaceutical materials.

The study was carried out using either sex of Swiss albino mice (20-25 g) & wistar rats (200-250g). The methanolic extract of Holarrhena antidysenterica was investigated for analgesic activity using hot plate method & Tail Flick method. The ME of Holarrhena antidysenterica bark was used in pain model. The analgesic activity of Holarrhena antidysenterica at the dose of (200, 400, 600 mg/kg p.o) showed significantly (p<0.05) increase in mean basal reaction time in Hot plate method & increase in latency to flick tail (p<0.05) compared with control group of animals. The ME of the Holarrhena antidysenterica has significant analgesic activity in mice & rats.

A simple and reproducible High Performance Thin Layer Chromatographic method for the determination of bacoside A in Bacopa monnieri and its prepared formulations was developed and is described. Phytochemical screening of methanolic extract of Bacopa monnieri is performed and a Thin Layer Chromatographic method was developed using n-Butanol: Acetic acid: Water (36: 6: 8 v/v). The High Performance Thin Layer Chromatographic method involved separation of components by TLC on stationary phase i.e. precoated silica gel GF254 with a solvent system of n-butanol: Acetic acid: Water (36:6:8) and detection was carried out by scanning and quantifying the peak at 580 nm for bacoside A. The method was validated in terms of linearity, accuracy and precision. The sensitivity of High Performance Thin Layer Chromatographic method was found to be 46 ng and the linearity was observed in the range of 200 ng to 1200 ng. The proposed method was found precise and sensitive and can be used for detection, monitoring and quantification of bacoside A in B. monnieri and its formulations.

The root tubers of Chlorophytum borivilianum Sant & Fern (Safed Musli) (Liliaceae) are reported to have a greater medicinal value. Pharmacognostic evaluation including examination of morphological and microscopical characters, determination of ash values, swelling & foaming index, extractive values, moisture content and fluorescence characteristics of powder drug with different chemical reagents was carried out.

The current study was to investigate the presence of Anti-Bacterial activity on the different root extracts of Mimosa rubicaulis Lam.. The extracts obtained using continuous hot percolation method and Anti-Bacterial activity tested by using cup plate method. The present investigation reveals that all the three extracts namely ethylacetate, methanol and water extracts of concentration 1000 mcg/ml showing Anti-Bacterial activity against both gram + ve and gram –ve organisms. Among all the three extracts root methanolic extract showing more anti-bacterial activity. The study shown that the all the three root extracts of Mimosa rubicaulis Lam. Possess Anti-Bacterial activity.