Sejal S. Rathod, Shailju Gurunani, Sonal Motghare
Uma Maheswari Kolipaka, A. Sumana Sri, Sk. Karishma, G. Vinay Kumar Reddy, B. Devi Somya Sri, R. Gowri Prasad Singh, I. Siva Sudhakar Reddy
The current research explored the antibacterial potential of a non-polar solvent extract from the seeds of four citrus fruit varieties against Lactobacillus species. An ethanol and diethyl ether extract was prepared through a 72-hour soaking process, followed by separation of its components using thin-layer chromatography (TLC) and liquid column chromatography. Phytochemical analysis of the isolated fractions revealed the presence of phenols, flavonoids, and terpenoids. Antibacterial activity was evaluated using the cup plate method, which showed a 15mm inhibition zone for the initial extract against the tested Lactobacillus species. These findings indicate that the non-polar extract of citrus fruit seeds contains active compounds with antibacterial properties, suggesting a need for further research into its potential use in developing new antimicrobial agents.
A B Kadam