Anti oxidant, anti inflammatory, anti obesity and anti microbial activities in four different species of Caralluma, include Boucerosea lasiantha (BL), Caralluma adscendens var. attenuata (CAA), C.stalagmifera (CSF) and C.longipetala (CL) were investigated. Whole plant is used for the extraction of compound by using methanol. These selected species of Caralluma are used for the study of saponins and flavanoids, their bioactive nature. Variations in the bioactivity of cactus species are compared as a preliminary survey for future pharmacological drug development. Methanolic extracts of CAA exhibited highest free radical scavenger activity (FRSA) followed by BL, CSF, CL respectively with IC50 values 50,37,32, 27 μg/ml. CAA also exhibited highest anti inflammatory activity (5-Lox assay) followed by BL, CL, CSF with IC50 values 27 ,17, 12.8, 11.8 μg/ml. Methanolic extracts of four species also show anti diabetic activities (α- amylase property assay). Extracts of four species show anti microbial activity, however CSF and CL show highest activity than CAA and BL. These results suggest that the methanol extract of Caralluma species show significant anti oxidant, anti inflammatory, anti diabetic, anti microbial activities, which could be used as a potential source of pharmaceutical materials.